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Bioinformatics Analysis and Validation of the Expressed Sequences Tag in Human Colorectal Adenocarcinoma
Abstract
Background: This study was to investigate some new pathological genes in colorectal adenocarcinoma of human.
Methods: Human colorectal adenocarcinoma tissues and normal colorectal tissues were taken and suppression subtractive hybridization (SSH) and cDNA microarray techniques were employed. From differentially expressed 86 expressed sequence tags (EST), 10 EST of the SSH were selected as seed sequence for bioinformatics analyses, semi-quantitative RT-PCR and PCR-sequencing . Each lane of semi-quantitative RT-PCR was analyzed by Q1 software.
Results: Among these 10 EST, it has been found that ES274070, ES274071, ES274076 and ES274081 may play role in the onset of colorectal adenocarcinoma in human.
Conclusions: The ES274070, ES274071, ES274076 and ES274081 are related to the onset of human colorectal adenocarcinoma.
Gastroenterol Res. 2009;2(2):110-114
doi: https://doi.org/10.4021/gr2009.04.1287
Methods: Human colorectal adenocarcinoma tissues and normal colorectal tissues were taken and suppression subtractive hybridization (SSH) and cDNA microarray techniques were employed. From differentially expressed 86 expressed sequence tags (EST), 10 EST of the SSH were selected as seed sequence for bioinformatics analyses, semi-quantitative RT-PCR and PCR-sequencing . Each lane of semi-quantitative RT-PCR was analyzed by Q1 software.
Results: Among these 10 EST, it has been found that ES274070, ES274071, ES274076 and ES274081 may play role in the onset of colorectal adenocarcinoma in human.
Conclusions: The ES274070, ES274071, ES274076 and ES274081 are related to the onset of human colorectal adenocarcinoma.
Gastroenterol Res. 2009;2(2):110-114
doi: https://doi.org/10.4021/gr2009.04.1287
Keywords
Bioinformatic analyses; Expressed sequence tag; Colorectal adenocarcinoma