Gastroenterology Research, ISSN 1918-2805 print, 1918-2813 online, Open Access
Article copyright, the authors; Journal compilation copyright, Gastroenterol Res and Elmer Press Inc
Journal website http://www.gastrores.org

Original Article

Volume 11, Number 1, February 2018, pages 46-51


Analysis of Helicobacter pylori Genotypes Amongst Jordanians’ Dental Plaque Samples

Figure

Figure 1.
Figure 1. PCR genotyping of vacA and cagA genes from the volunteers’ samples (lanes- 1 = 50 bp molecular weight marker; 2 = cagA+; 3 = S1a; 4 = S1b; 5 = S2; 6 = m1; 7 = m2 and 8 = negative control (without DNA)).

Tables

Table 1. PCR Primers for Amplification of cagA and vacA Genes
 
RegionPrimerPrimer sequencePCR product size (bp)
cagAF1GAGCAATCGCTTACGCTCAG250
R1GTGAATGGAACCCTAGTCGG
vacA
  m1VA3-FGGTCAAAATGCGGTCATGG290
VA3-RCCATTGGTACCTGTAGAAAC
  m2VA4-FGGAGCCCCAGGAAACATTG352
VA4-RCATAACTAGCGCCTTGCAC
  s1bSS3-RAGCGCCATACCGCAAGAG187
VA1-RCTGCTTGAATGCGCCAAAC
  s1aSS1-RGTCAGCATCACACCGCAAC190
VA1-RCTGCTTGAATGCGCCAAAC
  s2SS2-RGCTAACACGCCAAATGATGC199
VA1-RCTGCTTGAATGCGCCAAAC

 

Table 2. Prevalence of cagA Gene, vacA Alleles, and vacA Allele Combinations
 
Gene statusNumber of samples (%)
cagA
  cagA+14 (23.3)
  cagA-46 (76.7)
vacA
  m19 (15)
  m254 (90)
  s1a33 (55)
  s1b32 (53.3)
  s230 (50)
  m1/m24 (6.6)
  s1a/m14 (6.6)
  s1b/m17 (11.6)
  s1a/m229 (48.3)
  s1b/m227 (45)
  s2/m16 (10)
  s2/m227 (45)