Figure 1. PCR genotyping of vacA and cagA genes from the volunteers’ samples (lanes- 1 = 50 bp molecular weight marker; 2 = cagA+; 3 = S1a; 4 = S1b; 5 = S2; 6 = m1; 7 = m2 and 8 = negative control (without DNA)).
| Gastroenterology Research, ISSN 1918-2805 print, 1918-2813 online, Open Access |
| Article copyright, the authors; Journal compilation copyright, Gastroenterol Res and Elmer Press Inc |
| Journal website http://www.gastrores.org |
Original Article
Volume 11, Number 1, February 2018, pages 46-51
Analysis of Helicobacter pylori Genotypes Amongst Jordanians’ Dental Plaque Samples
Figure
Tables
| Region | Primer | Primer sequence | PCR product size (bp) |
|---|---|---|---|
| cagA | F1 | GAGCAATCGCTTACGCTCAG | 250 |
| R1 | GTGAATGGAACCCTAGTCGG | ||
| vacA | |||
| m1 | VA3-F | GGTCAAAATGCGGTCATGG | 290 |
| VA3-R | CCATTGGTACCTGTAGAAAC | ||
| m2 | VA4-F | GGAGCCCCAGGAAACATTG | 352 |
| VA4-R | CATAACTAGCGCCTTGCAC | ||
| s1b | SS3-R | AGCGCCATACCGCAAGAG | 187 |
| VA1-R | CTGCTTGAATGCGCCAAAC | ||
| s1a | SS1-R | GTCAGCATCACACCGCAAC | 190 |
| VA1-R | CTGCTTGAATGCGCCAAAC | ||
| s2 | SS2-R | GCTAACACGCCAAATGATGC | 199 |
| VA1-R | CTGCTTGAATGCGCCAAAC |
| Gene status | Number of samples (%) |
|---|---|
| cagA | |
| cagA+ | 14 (23.3) |
| cagA- | 46 (76.7) |
| vacA | |
| m1 | 9 (15) |
| m2 | 54 (90) |
| s1a | 33 (55) |
| s1b | 32 (53.3) |
| s2 | 30 (50) |
| m1/m2 | 4 (6.6) |
| s1a/m1 | 4 (6.6) |
| s1b/m1 | 7 (11.6) |
| s1a/m2 | 29 (48.3) |
| s1b/m2 | 27 (45) |
| s2/m1 | 6 (10) |
| s2/m2 | 27 (45) |