Gastroenterology Research, ISSN 1918-2805 print, 1918-2813 online, Open Access
Article copyright, the authors; Journal compilation copyright, Gastroenterol Res and Elmer Press Inc
Journal website http://www.gastrores.org

Original Article

Volume 5, Number 6, December 2012, pages 227-231

A Modified Perfusion Method to Improve the Quality of Procured Donor Pancreas in Rats

Figures

Figure 1.
Figure 1. In study group pancreatic acinar structures were normal, and islets were clearly observed located in the center of lobule with normal size. H&E staining, 10 × 10.
Figure 2.
Figure 2. In control group, the pancreatic lobule interstitial space was widening, loose, and edematous. Islets were slightly shrinked with scattered lymphocytes infiltration in the lobules. H&E staining, 10 × 10.
Figure 3.
Figure 3. Immunohistochemistry of pancreas in the control group, P-selectin was mildly expressed. 10 × 40.
Figure 4.
Figure 4. Immunohistochemistry of pancreas in the control group, ICAM-1 was strongly expressed. 10 × 10.
Figure 5.
Figure 5. Immunohistochemistry of pancreas in the study group, no P-selectin was expressed. 10 × 20.
Figure 6.
Figure 6. Immunohistochemistry of pancreas in the study group, ICAM-1 was mildly expressed. 10 × 10.

Tables

Table 1. Histological Examinations (H&E staining)
 
TimeGroupVenous congestionInterstitial edemaInflammation cell infiltration
Negative (n)Positive (n)Negative (n)Positive (n)Negative (n)Positive (n)
* Compared with control group, Fisher exact probability test, P < 0.05; ** Compared with control group, Chi-square test, P < 0.05.
6h* post-perfusionStudy240240240
Control195177204
10h** post-perfusionStudy222213231
Control111310141014
14h** post-perfusionStudy213204204
Control717618321

 

Table 2. Immunohistochemistry (P-Selectin)
 
TimeGroupNegative (n)Mild Positive (n)Positive (n)
* Compared with control group, Fisher exact probability test, P > 0.05; ** Compared with control group, Chi-square test, P < 0.05.
6h* post-perfusionStudy2400
Control2031
10h** post-perfusionStudy2400
Control1752
14h** post-perfusionStudy2220
Control1284

 

Table 3. Immunohistochemistry (ICAM-1)
 
TimeGroupNegative (n)Mild Positive (n)Positive (n)
* Compared with control group, Fisher exact probability test, P < 0.05; ** Compared with control group, Chi-square test, P < 0.05.
6h* post-perfusionStudy2400
Control1455
10h** post-perfusionStudy2310
Control1167
14h** post-perfusionStudy2031
Control7710