Gastroenterology Research, ISSN 1918-2805 print, 1918-2813 online, Open Access
Article copyright, the authors; Journal compilation copyright, Gastroenterol Res and Elmer Press Inc
Journal website http://www.gastrores.org

Original Article

Volume 5, Number 3, June 2012, pages 85-96


Ischaemic Preconditioning and Intermittent Clamping Does not Influence Mediators of Liver Regeneration in a Human Liver Sinusoidal Endothelial Cell Model of Ischaemia-Reperfusion Injury

Figures

Figure 1.
Figure 1. Ischaemia and reperfusion protocols used in this study.
Figure 2.
Figure 2. Human liver SECs. (a) Appearance of SECs at day 1 of extraction (phase contrast x 100).
Figure 3.
Figure 3. Immunohistochemistry demonstrating the expression of CD31 in human liver SECs (fluorescence x 200).
Figure 4.
Figure 4. Immunohistochemistry demonstrating the expression of vWF in SECs. Phase contrast image of the same field of view is shown for comparison. (a) Phase contrast x 200. (b) Fluorescence x 200.
Figure 5.
Figure 5. Fluorescent image demonstrating the uptake of FITC-FSA in human liver SECs. Phase contrast image of the same field of view is shown for comparison. (a) Phase contrast x 200. (b) Fluorescence x 200.

Tables

Table 1. Production Level of IL-6 by SECs That Underwent IRI, IPC and IC at Their Respective Time Points
 
Time (Hours)Control (Mean (SEM) pg/mL)IRIIPCIC
Mean (SEM) pg/mLP-valueξMean (SEM) pg/mLP-valueβMean (SEM) pg/mLP-valueχ
ξ: P-value was derived from Mann Whitney U test between the IRI and control groups. β: P-value was derived from Mann Whitney U test between the IPC and IRI groups. χ: P-value was derived from Mann Whitney U test between the IC and IRI groups. NS: Not significant.
04.14 (1.49)7.03 (1.57)NS6.81 (1.61)NS5.82 (1.25)NS
410.28 (1.24)30.34 (7.39)0.02837.84 (9.39)NS29.28 (7.35)NS
838.18 (9.90)60.36 (11.74)NS66.79 (11.51)NS69.85 (23.62)NS
1245.49 (11.25)73.14 (14.75)NS77.72 (14.71)NS75.03 (17.78)NS
2499.90 (35.02)156.80 (43.62)NS139.68 (34.62)NS156.50 (42.78)NS
36180.62 (63.74)253.83 (86.50)NS334.22 (145.38)NS309.92 (106.77)NS
48310.05 (126.36)449.89 (135.11)NS572.22 (139.44)NS662.78 (149.64)NS

 

Table2. Production Level of IL-8 by SECs That Underwent IRI, IPC and IC at Their Respective Time Points
 
Time (Hours)Control (Mean (SEM) pg/mL)IRIIPCIC
Mean (SEM) pg/mLP-valueξMean (SEM) pg/mLP-valueβMean (SEM) pg/mLP-valueχ
ξ: P-value was derived from Mann Whitney U test between the IRI and control groups. β: P-value was derived from Mann Whitney U test between the IPC and IRI groups. χ: P-value was derived from Mann Whitney U test between the IC and IRI groups. NS: Not significant.
0108.78 (26.72)118.58 (18.76)NS99.63 (18.34)NS100.43 (21.17)NS
4384.67 (95.76)888.47 (332.47)NS885.73 (281.69)NS830.10 (262.55)NS
8993.77 (303.52)1836.53 (750.05)NS1833.00 (812.49)NS1658.43 (552.36)NS
121480.26 (630.49)2318.59 (926.13)NS2318.41 (873.36)NS2244.22 (769.89)NS
242339.97 (866.46)3650.34 (1179.0)NS3727.84 (308.26)NS3811.90 (1282.7)NS
36495.56 (177.68)528.10 (161.22)NS613.32 (178.73)NS695.27 (172.94)NS
48686.15 (336.40)763.70 (366.15)NS572.22 (139.44)NS842.01 (238.24)NS

 

Table 3. Production Level of G-CSF by SECs That Underwent IRI, IPC and IC at Their Respective Time Points
 
Time (Hours)Control (Mean (SEM) pg/mL)IRIIPCIC
Mean (SEM) pg/mLP-valueξMean (SEM) pg/mLP-valueβMean (SEM) pg/mLP-valueχ
ξ: P-value was derived from Mann Whitney U test between the IRI and control groups. β: P-value was derived from Mann Whitney U test between the IPC and IRI groups. χ: P-value was derived from Mann Whitney U test between the IC and IRI groups. NS: Not significant.
03.79 (1.25)7.53 (1.55)NS6.73 (1.20)NS4.44 (0.60)NS
411.44 (5.91)25.67 (5.53)NS22.47 (6.65)NS19.90 (5.23)NS
840.61 (17.39)74.36 (22.74)NS67.11 (23.69)NS63.75 (20.32)NS
1270.74 (31.93)108.61 (31.76)NS92.37 (26.42)NS101.80 (28.18)NS
24159.52 (65.94)234.59 (73.61)NS208.74 (59.72)NS243.60 (63.22)NS
36214.30 (88.84)330.58 (99.61)NS392.03 (133.34)NS432.04 (152.23)NS
48306.84 (116.61)467.89 (128.72)NS656.95 (253.13)NS719.84 (268.52)NS

 

Table 4. Production Level of HGF by SECs That Underwent IRI, IPC and IC at Their Respective Time Points
 
Time (Hours)Control (Mean (SEM) pg/mL)IRIIPCIC
Mean (SEM) pg/mLP-valueξMean (SEM) pg/mLP-valueβMean (SEM) pg/mLP-valueχ
ξ: P-value was derived from Mann Whitney U test between the IRI and control groups. β: P-value was derived from Mann Whitney U test between the IPC and IRI groups. χ: P-value was derived from Mann Whitney U test between the IC and IRI groups. NS: Not significant.
02.57 (0.76)4.40 (0.56)NS4.74 (1.83)NS6.58 (2.36)NS
46.36 (2.83)13.40 (2.44)NS19.34 (7.34)NS21.88 (5.53)NS
816.86 (6.58)25.36 (5.06)NS43.28 (16.26)NS34.04 (11.41)NS
1215.45 (3.19)35.56 (8.15)NS49.16 (16.99)NS47.00 (12.76)NS
2428.65 (8.87)48.28 (11.38)NS60.82 (23.02)NS83.95 (25.26)NS
3625.73 (8.20)62.64 (12.96)NS119.69 (26.44)NS132.00 (11.89)NS
48110.16 (50.80)171.78 (50.19)NS182.70 (39.07)NS208.52 (27.50)NS