Gastroenterol Res

Gastroenterology Research, ISSN 1918-2805 print, 1918-2813 online, Open Access

Article copyright, the authors; Journal compilation copyright, Gastroenterol Res and Elmer Press Inc

Journal website http://www.gastrores.org

Original Article

Volume 5, Number 3, June 2012, pages 85-96

Ischaemic Preconditioning and Intermittent Clamping Does not Influence Mediators of Liver Regeneration in a Human Liver Sinusoidal Endothelial Cell Model of Ischaemia-Reperfusion Injury

Figure 4. Immunohistochemistry demonstrating the expression of vWF in SECs. Phase contrast image of the same field of view is shown for comparison. (a) Phase contrast x 200. (b) Fluorescence x 200.

Figure 5. Fluorescent image demonstrating the uptake of FITC-FSA in human liver SECs. Phase contrast image of the same field of view is shown for comparison. (a) Phase contrast x 200. (b) Fluorescence x 200.

**Table 1.** Production Level of IL-6 by SECs That Underwent IRI, IPC and IC at Their Respective Time Points
Time (Hours)	Control (Mean (SEM) pg/mL)	IRI	IPC	IC
^ξ: P-value was derived from Mann Whitney U test between the IRI and control groups. ^β: P-value was derived from Mann Whitney U test between the IPC and IRI groups. ^χ: P-value was derived from Mann Whitney U test between the IC and IRI groups. NS: Not significant.
0	4.14 (1.49)	7.03 (1.57)	NS	6.81 (1.61)	NS	5.82 (1.25)	NS
4	10.28 (1.24)	30.34 (7.39)	0.028	37.84 (9.39)	NS	29.28 (7.35)	NS
8	38.18 (9.90)	60.36 (11.74)	NS	66.79 (11.51)	NS	69.85 (23.62)	NS
12	45.49 (11.25)	73.14 (14.75)	NS	77.72 (14.71)	NS	75.03 (17.78)	NS
24	99.90 (35.02)	156.80 (43.62)	NS	139.68 (34.62)	NS	156.50 (42.78)	NS
36	180.62 (63.74)	253.83 (86.50)	NS	334.22 (145.38)	NS	309.92 (106.77)	NS
48	310.05 (126.36)	449.89 (135.11)	NS	572.22 (139.44)	NS	662.78 (149.64)	NS

Table 1. Production Level of IL-6 by SECs That Underwent IRI, IPC and IC at Their Respective Time Points

Time (Hours)

Control (Mean (SEM) pg/mL)

IRI

IPC

Mean (SEM) pg/mL

P-value^ξ

Mean (SEM) pg/mL

P-value^β

Mean (SEM) pg/mL

P-value^χ

^ξ: P-value was derived from Mann Whitney U test between the IRI and control groups. ^β: P-value was derived from Mann Whitney U test between the IPC and IRI groups. ^χ: P-value was derived from Mann Whitney U test between the IC and IRI groups. NS: Not significant.

4.14 (1.49)

7.03 (1.57)

6.81 (1.61)

5.82 (1.25)

10.28 (1.24)

30.34 (7.39)

0.028

37.84 (9.39)

29.28 (7.35)

38.18 (9.90)

60.36 (11.74)

66.79 (11.51)

69.85 (23.62)

45.49 (11.25)

73.14 (14.75)

77.72 (14.71)

75.03 (17.78)

99.90 (35.02)

156.80 (43.62)

139.68 (34.62)

156.50 (42.78)

180.62 (63.74)

253.83 (86.50)

334.22 (145.38)

309.92 (106.77)

310.05 (126.36)

449.89 (135.11)

572.22 (139.44)

662.78 (149.64)

**Table2.** Production Level of IL-8 by SECs That Underwent IRI, IPC and IC at Their Respective Time Points
Time (Hours)	Control (Mean (SEM) pg/mL)	IRI	IPC	IC
^ξ: P-value was derived from Mann Whitney U test between the IRI and control groups. ^β: P-value was derived from Mann Whitney U test between the IPC and IRI groups. ^χ: P-value was derived from Mann Whitney U test between the IC and IRI groups. NS: Not significant.
0	108.78 (26.72)	118.58 (18.76)	NS	99.63 (18.34)	NS	100.43 (21.17)	NS
4	384.67 (95.76)	888.47 (332.47)	NS	885.73 (281.69)	NS	830.10 (262.55)	NS
8	993.77 (303.52)	1836.53 (750.05)	NS	1833.00 (812.49)	NS	1658.43 (552.36)	NS
12	1480.26 (630.49)	2318.59 (926.13)	NS	2318.41 (873.36)	NS	2244.22 (769.89)	NS
24	2339.97 (866.46)	3650.34 (1179.0)	NS	3727.84 (308.26)	NS	3811.90 (1282.7)	NS
36	495.56 (177.68)	528.10 (161.22)	NS	613.32 (178.73)	NS	695.27 (172.94)	NS
48	686.15 (336.40)	763.70 (366.15)	NS	572.22 (139.44)	NS	842.01 (238.24)	NS

Table2. Production Level of IL-8 by SECs That Underwent IRI, IPC and IC at Their Respective Time Points

Time (Hours)

Control (Mean (SEM) pg/mL)

IRI

IPC

Mean (SEM) pg/mL

P-value^ξ

Mean (SEM) pg/mL

P-value^β

Mean (SEM) pg/mL

P-value^χ

108.78 (26.72)

118.58 (18.76)

99.63 (18.34)

100.43 (21.17)

384.67 (95.76)

888.47 (332.47)

885.73 (281.69)

830.10 (262.55)

993.77 (303.52)

1836.53 (750.05)

1833.00 (812.49)

1658.43 (552.36)

1480.26 (630.49)

2318.59 (926.13)

2318.41 (873.36)

2244.22 (769.89)

2339.97 (866.46)

3650.34 (1179.0)

3727.84 (308.26)

3811.90 (1282.7)

495.56 (177.68)

528.10 (161.22)

613.32 (178.73)

695.27 (172.94)

686.15 (336.40)

763.70 (366.15)

572.22 (139.44)

842.01 (238.24)

**Table 3.** Production Level of G-CSF by SECs That Underwent IRI, IPC and IC at Their Respective Time Points
Time (Hours)	Control (Mean (SEM) pg/mL)	IRI	IPC	IC
^ξ: P-value was derived from Mann Whitney U test between the IRI and control groups. ^β: P-value was derived from Mann Whitney U test between the IPC and IRI groups. ^χ: P-value was derived from Mann Whitney U test between the IC and IRI groups. NS: Not significant.
0	3.79 (1.25)	7.53 (1.55)	NS	6.73 (1.20)	NS	4.44 (0.60)	NS
4	11.44 (5.91)	25.67 (5.53)	NS	22.47 (6.65)	NS	19.90 (5.23)	NS
8	40.61 (17.39)	74.36 (22.74)	NS	67.11 (23.69)	NS	63.75 (20.32)	NS
12	70.74 (31.93)	108.61 (31.76)	NS	92.37 (26.42)	NS	101.80 (28.18)	NS
24	159.52 (65.94)	234.59 (73.61)	NS	208.74 (59.72)	NS	243.60 (63.22)	NS
36	214.30 (88.84)	330.58 (99.61)	NS	392.03 (133.34)	NS	432.04 (152.23)	NS
48	306.84 (116.61)	467.89 (128.72)	NS	656.95 (253.13)	NS	719.84 (268.52)	NS

Table 3. Production Level of G-CSF by SECs That Underwent IRI, IPC and IC at Their Respective Time Points

Time (Hours)

Control (Mean (SEM) pg/mL)

IRI

IPC

Mean (SEM) pg/mL

P-value^ξ

Mean (SEM) pg/mL

P-value^β

Mean (SEM) pg/mL

P-value^χ

3.79 (1.25)

7.53 (1.55)

6.73 (1.20)

4.44 (0.60)

11.44 (5.91)

25.67 (5.53)

22.47 (6.65)

19.90 (5.23)

40.61 (17.39)

74.36 (22.74)

67.11 (23.69)

63.75 (20.32)

70.74 (31.93)

108.61 (31.76)

92.37 (26.42)

101.80 (28.18)

159.52 (65.94)

234.59 (73.61)

208.74 (59.72)

243.60 (63.22)

214.30 (88.84)

330.58 (99.61)

392.03 (133.34)

432.04 (152.23)

306.84 (116.61)

467.89 (128.72)

656.95 (253.13)

719.84 (268.52)

**Table 4.** Production Level of HGF by SECs That Underwent IRI, IPC and IC at Their Respective Time Points
Time (Hours)	Control (Mean (SEM) pg/mL)	IRI	IPC	IC
^ξ: P-value was derived from Mann Whitney U test between the IRI and control groups. ^β: P-value was derived from Mann Whitney U test between the IPC and IRI groups. ^χ: P-value was derived from Mann Whitney U test between the IC and IRI groups. NS: Not significant.
0	2.57 (0.76)	4.40 (0.56)	NS	4.74 (1.83)	NS	6.58 (2.36)	NS
4	6.36 (2.83)	13.40 (2.44)	NS	19.34 (7.34)	NS	21.88 (5.53)	NS
8	16.86 (6.58)	25.36 (5.06)	NS	43.28 (16.26)	NS	34.04 (11.41)	NS
12	15.45 (3.19)	35.56 (8.15)	NS	49.16 (16.99)	NS	47.00 (12.76)	NS
24	28.65 (8.87)	48.28 (11.38)	NS	60.82 (23.02)	NS	83.95 (25.26)	NS
36	25.73 (8.20)	62.64 (12.96)	NS	119.69 (26.44)	NS	132.00 (11.89)	NS
48	110.16 (50.80)	171.78 (50.19)	NS	182.70 (39.07)	NS	208.52 (27.50)	NS

Table 4. Production Level of HGF by SECs That Underwent IRI, IPC and IC at Their Respective Time Points

Time (Hours)

Control (Mean (SEM) pg/mL)

IRI

IPC

Mean (SEM) pg/mL

P-value^ξ

Mean (SEM) pg/mL

P-value^β

Mean (SEM) pg/mL

P-value^χ

2.57 (0.76)

4.40 (0.56)

4.74 (1.83)

6.58 (2.36)

6.36 (2.83)

13.40 (2.44)

19.34 (7.34)

21.88 (5.53)

16.86 (6.58)

25.36 (5.06)

43.28 (16.26)

34.04 (11.41)

15.45 (3.19)

35.56 (8.15)

49.16 (16.99)

47.00 (12.76)

28.65 (8.87)

48.28 (11.38)

60.82 (23.02)

83.95 (25.26)

25.73 (8.20)

62.64 (12.96)

119.69 (26.44)

132.00 (11.89)

110.16 (50.80)

171.78 (50.19)

182.70 (39.07)

208.52 (27.50)