Gastroenterol Res

Gastroenterology Research, ISSN 1918-2805 print, 1918-2813 online, Open Access

Article copyright, the authors; Journal compilation copyright, Gastroenterol Res and Elmer Press Inc

Journal website http://www.gastrores.org

Original Article

Volume 4, Number 1, February 2011, pages 13-19

Significance of Serum Alpha-Glutathione S-Transferase Assessment in Hepatitis C Patients with Different Alanine Aminotransferase Patterns

**Table 1.** Comparison Between the Mean ± SD of HCV RNA, ALT, AST, ALP, GGT, TP, Alb, and α-GST Levels in the Study Groups.
	Control (n = 21)	NALT (n = 12)	NNALT (n = 29)	HALT (n = 24)	All HCV (n = 65)
P: comparison between various patients groups and control. NALT: normal ALT group (ALT < 40 IU/L); NNALT: near normal ALT group (40 - 80 IU/L); HALT: high ALT group (ALT > 80 IU/L); α-GST: Alpha-Glutathione S-transferase; n: number; ALT: alanine aminotransferase; AST: aspartate aminotransferase; ALP: alkaline phosphatase; GGT: gamma glutamyl-transferase; TP: total proteins; Alb: albumin; HCV: hepatitis C virus.
HCV RNA (IU/ml (x10⁴)) P	0.00	1.56 ± 2.8 0.000	16.71 ± 23.3 0.000	32.38 ± 40.5 0.000	19.70 ± 30.9 0.000
ALT (IU/L) P	25.29 ± 7.1	30.08 ± 5.8 0.514	61.45 ± 11.5 0.028	132.42 ± 53.3 < 0.001	81.86 ± 52.3 < 0.001
AST (IU/L) P	24.26 ± 6.6	27.75 ± 5.1 0.384	45.45 ± 9.5 0.035	97.92 ± 46.7 < 0.001	61.55 ± 40.7 < 0.001
ALP (IU/L) P	164.52 ± 40.9	171.25 ± 40.3 1.000	191.62 ± 41.2 0.991	225.17 ± 65.1 0.040	200.25 ± 54.5 < 0.001
GGT (IU/L) P	32.86 ± 8.2	34.42 ± 8.5 0.872	47.69 ± 14.6 0.048	82.21 ± 40.7 < 0.001	57.99 ± 32.8 < 0.001
TP (g/dl) P	7.40 ± 0.5	7.10 ± 0.5 0.920	6.69 ± 0.6 0.521	6.52 ± 0.5 0.972	6.71 ± 0.6 0.602
Alb (g/dl) P	4.61 ± 0.6	4.23 ± 0.6 0.947	3.86 ± 0.5 0.607	3.45 ± 0.5 0.246	3.78 ± 0.6 1.038
α-GST (ng/ml) P	0.27 ± 2.1	10.51 ± 3.4 0.019	16.25 ± 7.7 < 0.001	18.56 ± 9.7 < 0.001	16.04 ± 8.4 < 0.001

Table 1. Comparison Between the Mean ± SD of HCV RNA, ALT, AST, ALP, GGT, TP, Alb, and α-GST Levels in the Study Groups.

Control (n = 21)

NALT (n = 12)

NNALT (n = 29)

HALT (n = 24)

All HCV (n = 65)

P: comparison between various patients groups and control. NALT: normal ALT group (ALT < 40 IU/L); NNALT: near normal ALT group (40 - 80 IU/L); HALT: high ALT group (ALT > 80 IU/L); α-GST: Alpha-Glutathione S-transferase; n: number; ALT: alanine aminotransferase; AST: aspartate aminotransferase; ALP: alkaline phosphatase; GGT: gamma glutamyl-transferase; TP: total proteins; Alb: albumin; HCV: hepatitis C virus.

HCV RNA (IU/ml (x10⁴)) P

0.00

1.56 ± 2.8 0.000

16.71 ± 23.3 0.000

32.38 ± 40.5 0.000

19.70 ± 30.9 0.000

ALT (IU/L) P

25.29 ± 7.1

30.08 ± 5.8 0.514

61.45 ± 11.5 0.028

132.42 ± 53.3 < 0.001

81.86 ± 52.3 < 0.001

AST (IU/L) P

24.26 ± 6.6

27.75 ± 5.1 0.384

45.45 ± 9.5 0.035

97.92 ± 46.7 < 0.001

61.55 ± 40.7 < 0.001

ALP (IU/L) P

164.52 ± 40.9

171.25 ± 40.3 1.000

191.62 ± 41.2 0.991

225.17 ± 65.1 0.040

200.25 ± 54.5 < 0.001

GGT (IU/L) P

32.86 ± 8.2

34.42 ± 8.5 0.872

47.69 ± 14.6 0.048

82.21 ± 40.7 < 0.001

57.99 ± 32.8 < 0.001

TP (g/dl) P

7.40 ± 0.5

7.10 ± 0.5 0.920

6.69 ± 0.6 0.521

6.52 ± 0.5 0.972

6.71 ± 0.6 0.602

Alb (g/dl) P

4.61 ± 0.6

4.23 ± 0.6 0.947

3.86 ± 0.5 0.607

3.45 ± 0.5 0.246

3.78 ± 0.6 1.038

α-GST (ng/ml) P

0.27 ± 2.1

10.51 ± 3.4 0.019

16.25 ± 7.7 < 0.001

18.56 ± 9.7 < 0.001

16.04 ± 8.4 < 0.001

**Table 2.** Correlation Analysis Between α-GST and HCV RNA, ALT, AST, ALP, GGT, TP, and Alb Levels in the Study Groups.
	HCV RNA	ALT	AST	ALP	GGT	TP	Alb
NALT: normal ALT group (ALT < 40 IU/L); NNALT: near normal ALT group (40 - 80 IU/L); HALT: high ALT group (ALT > 80 IU/L); α-GST: Alpha-Glutathione S-transferase; n: number; ALT: alanine aminotransferase; AST: aspartate aminotransferase; ALP: alkaline phosphatase; GGT: gamma glutamyl-transferase; TP: total proteins; Alb: albumin; HCV: hepatitis C virus.
NALT	r	0.75	0.686	0.269	0.5005	0.562	- 0.322	- 0.265
	P	< 0.05	< 0.05	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
NNALT	r	0.523	0.763	0.737	0.499	0.698	- 0.443	- 0.419
	P	< 0.05	< 0.01	< 0.01	> 0.05	< 0.01	> 0.05	> 0.05
HALT	r	0.532	0.909	0.811	0.698	0.773	- 0.495	- 0.502
	P	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	> 0.05	> 0.05
ALL	r	0.645	0.685	0.657	0.549	0.594	- 0.443	- 0.419
HCV	P	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	> 0.05	> 0.05

Table 2. Correlation Analysis Between α-GST and HCV RNA, ALT, AST, ALP, GGT, TP, and Alb Levels in the Study Groups.

HCV RNA

ALT

AST

ALP

GGT

Alb

NALT: normal ALT group (ALT < 40 IU/L); NNALT: near normal ALT group (40 - 80 IU/L); HALT: high ALT group (ALT > 80 IU/L); α-GST: Alpha-Glutathione S-transferase; n: number; ALT: alanine aminotransferase; AST: aspartate aminotransferase; ALP: alkaline phosphatase; GGT: gamma glutamyl-transferase; TP: total proteins; Alb: albumin; HCV: hepatitis C virus.

NALT

0.75

0.686

0.269

0.5005

0.562

- 0.322

- 0.265

< 0.05

> 0.05

NNALT

0.523

0.763

0.737

0.499

0.698

- 0.443

- 0.419

< 0.05

< 0.01

> 0.05

< 0.01

> 0.05

HALT

0.532

0.909

0.811

0.698

0.773

- 0.495

- 0.502

< 0.01

> 0.05

ALL

0.645

0.685

0.657

0.549

0.594

- 0.443

- 0.419

HCV

< 0.01

> 0.05

**Table 3.** Sensitivity, Specificity, Positive Predictive Value and Negative Predictive Value of HCV RNA, ALT, AST, ALP, GGT, TP, Alb, and α-GST in all the HCV Patients Groups and Control.
	Sensitivity %	Specificity %	+ ve predictive	- ve predictive
n = number of cases.
HCV All Patients (n = 65)
HCV RNA	70.77	77.91	100.00	52.50
ALT	81.54	86.05	100.00	63.64
AST	64.62	73.26	100.00	47.73
ALP	9.23	31.40	100.00	26.25
GGT	46.15	59.30	100.00	37.50
TP	12.31	33.72	100.00	26.92
Alb	33.85	50.00	100.00	32.81
α-GST	81.54	84.88	98.15	62.50
NALT Group (n = 12)
HCV RNA	41.67	78.79	100.00	75.00
ALT	0.00	63.64	0.00	63.64
AST	0.00	63.64	0.00	63.64
ALP	0.00	63.64	0.00	63.64
GGT	0.00	63.64	0.00	63.64
TP	0.00	63.64	0.00	63.64
Alb	0.00	63.64	0.00	63.64
α-GST	66.67	84.85	88.89	83.33
NNALT Group (n = 29)
HCV RNA	75.8	86.00	100.00	75.00
ALT	100.00	100.00	100.00	100.00
AST	62.07	78.00	100.00	65.63
ALP	0.00	42.00	0.00	42.00
GGT	41.38	66.00	100.00	55.26
TP	13.79	50.00	100.00	45.65
Alb	27.59	58.00	100.00	50.00
α-GST	79.31	86.00	95.83	76.92
HALT Group (n = 24)
HCV RNA	79.17	88.89	100.00	80.77
ALT	100.00	100.00	100.00	100.00
AST	100.00	100.00	100.00	100.00
ALP	25.00	60.00	100.00	53.85
GGT	75.00	86.67	100.00	77.78
TP	16.67	55.56	100.00	51.22
Alb	58.33	77.78	100.00	67.74
α-GST	91.67	93.33	95.65	90.91

Table 3. Sensitivity, Specificity, Positive Predictive Value and Negative Predictive Value of HCV RNA, ALT, AST, ALP, GGT, TP, Alb, and α-GST in all the HCV Patients Groups and Control.

Sensitivity %

Specificity %

+ ve predictive

- ve predictive

Value%

n = number of cases.

HCV All Patients (n = 65)

HCV RNA

70.77

77.91

100.00

52.50

ALT

81.54

86.05

100.00

63.64

AST

64.62

73.26

100.00

47.73

ALP

9.23

31.40

100.00

26.25

GGT

46.15

59.30

100.00

37.50

12.31

33.72

100.00

26.92

Alb

33.85

50.00

100.00

32.81

α-GST

81.54

84.88

98.15

62.50

NALT Group (n = 12)

HCV RNA

41.67

78.79

100.00

75.00

ALT

0.00

63.64

0.00

63.64

AST

0.00

63.64

0.00

63.64

ALP

0.00

63.64

0.00

63.64

GGT

0.00

63.64

0.00

63.64

0.00

63.64

0.00

63.64

Alb

0.00

63.64

0.00

63.64

α-GST

66.67

84.85

88.89

83.33

NNALT Group (n = 29)

HCV RNA

75.8

86.00

100.00

75.00

ALT

100.00

AST

62.07

78.00

100.00

65.63

ALP

0.00

42.00

0.00

42.00

GGT

41.38

66.00

100.00

55.26

13.79

50.00

100.00

45.65

Alb

27.59

58.00

100.00

50.00

α-GST

79.31

86.00

95.83

76.92

HALT Group (n = 24)

HCV RNA

79.17

88.89

100.00

80.77

ALT

100.00

AST

100.00

ALP

25.00

60.00

100.00

53.85

GGT

75.00

86.67

100.00

77.78

16.67

55.56

100.00

51.22

Alb

58.33

77.78

100.00

67.74

α-GST

91.67

93.33

95.65

90.91