Gastroenterology Research, ISSN 1918-2805 print, 1918-2813 online, Open Access |

Article copyright, the authors; Journal compilation copyright, Gastroenterol Res and Elmer Press Inc |

Journal website http://www.gastrores.org |

Original Article

Volume 13, Number 3, June 2020, pages 107-113

**Evaluation of Roles of MicroRNA-21 and MicroRNA-18a in Esophageal Squamous Cell Carcinoma and Comparison of Their Changes in Expression Post-Chemoradiotherapy**

Figures

Tables

Variable | Patients (n = 30) | MiRNA-21 mean expression (RQ) ± SD | P value^{a} | MiRNA-18a mean expression (RQ) ± SD | P value^{b} |
---|---|---|---|---|---|

^{a}P value of miRNA-21; ^{b}P value of miRNA-18a. MiRNA: microRNA; RQ: relative quantification; SD: standard deviation. | |||||

Age | |||||

< 60 years | 20 | 65.31 ± 64.81 | 0.948 | 165.01 ± 289.59 | 0.746 |

≥ 60 years | 10 | 53.60 ± 33.45 | 72.52 ± 39.27 | ||

Sex | |||||

Male | 19 | 16.75 ± 17.34 | 0.445 | 30.28 ± 37.39 | 0.832 |

Female | 11 | 19.50 ± 14.58 | 50.19 ± 101.31 | ||

Risk factors | |||||

Smoking | |||||

Yes | 13 (43.33%) | 58.66 ± 61.219 | 0.68 | 37.61 ± 42.09 | 0.245 |

No | 17 (56.66%) | 63.51 ± 53.41 | 37.55 ± 82.65 | ||

Tobacco chewing | |||||

Yes | 15 (50%) | 14.82 ± 13.55 | 0.461 | 23.73 ± 35.83 | 0.267 |

No | 15 (50%) | 20.69 ± 18.44 | 51.43 ± 87.36 | ||

Alcohol | |||||

Yes | 7 | 21.35 ± 19.65 | 0.564 | 7.38 ± 6.62 | 0.61 |

No | 23 | 14.66 ± 13.11 | 4.15 ± 9.31 |

Variable | Patients (n = 30) | MiRNA-21 mean expression (RQ) ± SD | P value^{a, b} | MiRNA-18a mean expression (RQ) ± SD | P value^{a, c} |
---|---|---|---|---|---|

^{a}P values are from χ^{2} or Fisher’s exact probability test and were considered significant as 0.05. ^{b}P value of miRNA-21. ^{c}P value of miRNA-18a. ^{d}Clinical classification. ^{e}TNM classification. MiRNA: microRNA; RQ: relative quantification; SD: standard deviation. | |||||

Dysphagia | |||||

Grade 4 | 0 | - | 0.054 | - | 0.649 |

Grade 3 | 16 | 18.44 ± 13.03 | 34.31 ± 38.56 | ||

Grade 2 | 11 | 21.04 ± 20.43 | 19.21 ± 17.46 | ||

Grade 1 | 3 | 2.05 ± 1.25 | 122.38 ± 199.71 | ||

Tumor invasion | |||||

cT1^{d} | 5 | 990.93 ± 999.92 | 0.004 | 52.24 ± 88.27 | 0.743 |

cT2^{d} | 6 | 3,266.39 ± 5,282.25 | 16.52 ± 21.56 | ||

cT3^{d} | 15 | 5,738.60 ± 3,525.74 | 18.29 ± 21.63 | ||

cT4^{d} | 4 | 10,438.12 ± 5,129.27 | 8.29 ± 2.41 | ||

Lymph node metastasis | |||||

Absent | 10 | 3,655.72 ± 5,938.40 | 0.011 | 29.57 ± 63.63 | 0.502 |

Present | 20 | 5,791.36 ± 3,793.10 | 18.59 ± 21.20 | ||

Distant metastasis | |||||

Absent | 26 | 4,315.09 ± 4,005.00 | 0.038 | 23.16 ± 42.28 | 0.617 |

Present | 4 | 10,048.01 ± 5,950.24 | 16.34 ± 12.92 | ||

Stage^{e} | |||||

I, II | 10 | 2,247.64 ± 4,184.69 | 0.001 | 35.45 ± 63.40 | 0.846 |

III, IV | 20 | 6,495.40 ± 4,250.43 | 15.63 ± 19.18 | ||

Histology grading | |||||

Well-differentiated | 12 | 14.25 ± 13.02 | 0.439 | 44.62 ± 98.04 | 0.549 |

Moderately differentiated | 18 | 20.09 ± 17.95 | 32.89 ± 37.46 | ||

Poorly differentiated | 0 | - | - |

Variable | Area | SE^{a} | Asymptotic significance^{b} | 95% CI | |
---|---|---|---|---|---|

Lower bound | Upper bound | ||||

^{a}Under the nonparametric assumption. ^{b}Null hypothesis: true area = 0.5. AUC: area under the curve; ROC: receiver operating characteristic; SE: standard error; CI: confidence interval. | |||||

MiRNA-18a | 0.900 | 0.047 | 0.001 | 0.809 | 0.991 |

MiRNA-21 | 0.997 | 0.004 | 0.001 | 0.989 | 1.000 |